Somatic Cell Nuclear Transfer (SCNT)

Somatic Cell Nuclear Transfer (SCNT) is a technique in which the nucleus of a somatic (body) cell is transferred to the cytoplasm of an enucleated egg (an egg that has had its own nucleus removed).

  • Once inside the egg, the somatic nucleus is reprogrammed by egg cytoplasmic factors to become a zygote (fertilized egg) nucleus.
  • The egg is allowed to develop to the blastocyst stage, at which point a culture of embryonic stem cells (ESCs) can be created from the inner cell mass of the blastocyst.
  • Mouse, monkey, and human ESCs have been made using SCNT; human ESCs ....
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